Systematic Evaluation of the Immune Microenvironment of Neuroendocrine Tumors (NET)

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Introduction: Immunotherapy is being explored in many tumour types with encouraging results, but its role in the management of NET is yet to be defined.

Aim(s): Here we characterise the immune landscape of NET in order to inform a rational approach for immunotherapy in this patient group.

Materials and methods: Peripheral blood and fresh tissue (tumour and normal) were collected from consenting patients undergoing surgical resection for midgut NET. Multicolour flow-cytometry was performed to determine the abundance of CD8+, CD4+FoxP3- effector (CD4eff) and CD4+FoxP3+ regulatory (Treg) T cell subsets, and the expression of co-inhibitory and co-stimulatory checkpoint molecules. Matched tissue was obtained for immunohistochemistry (IHC) to investigate the geographical distribution of the immune infiltrate.

Conference: 15th Annual ENETSConcerence (2018)

Presenting Author: Vesely C

Authors: Vesely C, Childs A, Wong Y, Ogunbiyi O, Gander A,

Keywords: immunotherapy, neuroendocrine tumor, immune landscape, checkpoint molecules, t cells,