The Impact of Pharmacological-Mediated Depletion of Macrophages in Pancreatic Neuroendocrine Neoplasms

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Introduction: Pancreatic neuroendocrine tumors (PNETs) are a heterogeneous group of neuroendocrine neoplasms with distinct biological behaviour and response to treatment. Previous reports indicate that the infiltration of tumor-associated macrophages (TAMs) in PNETs strongly correlates with proliferation and metastases.

Aim(s): Evaluation of the impact of the liposomal clodronate (clodro) as pharmacological tool for macrophage depletion in-vitro and in-vivo.

Materials and methods: The effect of clodro on cell-viability was analysed in J774 and RAW myeloid cells and isolated murine bone macrophages as well as CD11b+ cells of RIP1-Tag2 pancreata which were evaluated by FACS. RIP1-Tag2 mice were treated with either clodro or liposomes alone to evaluate tumor progression, proliferation, angiogenesis and macrophage infiltration by FACS and immunohistochemistry.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author: Krug S

Authors: Krug S, Abbassi R, Rinke A, Griesmann H, Gress T,

Keywords: clodro, TAM, RIP1-Tag2,