The role of metabolism in neuroendocrine tumour cell models and patients

#4531

Introduction: It is recognised that the adipose tissue plays a major role in cancer through a metabolic coupling that supports tumour cell growth. Recent evidence suggests that altered metabolic pathways drive the development and influence the clinical outcomes of gastroenteropancreatic neuroendocrine tumours (GEP-NETs).

Aim(s): This study aims to explore the role of metabolism in NET cell models and patients.

Materials and methods: A retrospective study was conducted on 23 GEP-NET patients (11 ileal and 13 pancreatic). RNA-sequencing analysis was performed according to patient body mass index (BMI) (high BMI: >25; low BMI: <25) calculated from the clinical chart or extrapolated from PET/CT scan, using the tissue origin as a confounding factor. Indirect 3D co-culturing of adipocytes and pancreatic NET cell lines was performed using Transwell plates. At the end of the experiment, we performed triglyceride and metabolite assays on the culturing media and BODIPY staining for lipid droplet quantification on cultured cells.

Conference:

Presenting Author: Calabrese C

Authors: Calabrese C, Dellavalle S, Miserocchi G, Gabellone S, Spadazzi C,

Keywords: tumour metabolism, adipocytes, RNA-sequencing, GEP-NET,