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The Splicing Machinery Is Dysregulated in Low Grade Pulmonary Neuroendocrine Tumors


Introduction: Alternative splicing dysregulation is increasingly regarded as a novel cancer hallmark influencing all key tumor features. We have shown that neuroendocrine tumors (NETs) overexpress aberrantly spliced variants of neuropeptides and receptors that increase tumor malignancy. More recently, we discovered that the splicing machinery is dysregulated in pituitary and pancreatic NETs. To date, the status of this machinery in low grade pulmonary NETs is largely unknown.

Aim(s): To assess the expression profile of a selected set of components of the splicing machinery and explore their clinical relevance in low grade pulmonary NETs.

Materials and methods: A custom-made microfluidic based qPCR array was used to measure the expression of 45 key components of the splicing machinery in 33 pulmonary NETs samples (including typical and atypical carcinoids) compared to their non-tumoral adjacent tissue, and 5 healthy lung samples. Possible associations between gene expression levels and relevant clinical parameters were assessed.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Blázquez-Encinas R

Authors: Blázquez-Encinas R, Caro M, Pedraza-Arévalo S, Alors-Pérez E, Herrera-Martínez A,

Keywords: low grade pulmonary NETs, splicing, splicing machinery, malignancy,

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