TOPK promotes tumorigenesis and metastasis of pancreatic neuroendocrine neoplasms by enhancing the MAPK signalling pathway

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Introduction: T-LAK cell-originated protein kinase (TOPK) is highly expressed in pancreatic neuroendocrine neoplasms (pNENs) with liver metastasis and is associated with a poor prognosis.

Aim(s): The aim of this study is to elucidate the role and underlying mechanisms of TOPK in the progression and metastasis of pNENs. Additionally, the therapeutic potential of the TOPK inhibitor, HI-TOPK-032, is evaluated, providing a basis for potential targeted therapies in this challenging clinical context.

Materials and methods: To investigate the functional role of TOPK, knockdown experiments were performed in the BON-1 cell line, examining its effects on cell proliferation, epithelial-mesenchymal transition (EMT), and MAPK signalling pathway activation. RNA sequencing (RNA-seq) was conducted to analyse gene expression changes following TOPK knockdown. Additionally, the efficacy of HI-TOPK-032 in inhibiting pNENs cell proliferation, migration, and invasion was evaluated.

Conference:

Presenting Author: Chen Q

Authors: Chen Q, Yuan M, Meng M, Ren S, Li G,

Keywords: TOPK, Pancreatic Neuroendocrine Neoplasm, MAPK signalling pathway, Invasion and metastasis, HI-TOPK-032,