Transcriptomic analyses of multifocal ileal neuroendocrine tumors

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Introduction: Small intestinal neuroendocrine tumors are mostly located in the distal ileum with a high incidence of multiple synchronous primary tumors. To date, loss of heterozygosity (LOH) at chr18 has been the most frequent genomic event identified, occurring in ~60% of tumors. Recently, we showed that synchronous primary tumors from the same ileal NET patient display distinct somatic mutational profiles, including distinct patterns of chr18 LOH, suggesting these tumors originate independently. Thus, new mechanistic insights into multifocal ileal NETs are urgently needed.

Aim(s): To identify differentially expressed genes between multifocal ileal NETs, metastases and their matched normal ileum samples.

Materials and methods: Whole-transcriptome sequencing using ribosomal RNA depletion was performed for 68 synchronous primary tumors and 9 metastases, as well as their matched adjacent normal ileum from 11 patients with multifocal ileal NETs.

Conference:

Presenting Author:

Authors: Mäkinen N, Zhang Z, Munn S, Kasai Y, Kim G,

Keywords: Multifocal ileal NETs, RNA-sequencing, Gene expression,