Changes in DNA methylation are associated with differential Orthopedia Homeobox (OTP) expression in pulmonary carcinoids

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Introduction: Limited number of tumor types have been examined for Orthopedia Homeobox (OTP) expression. In pulmonary carcinoids, loss of expression is a strong indicator of poor prognosis.

Aim(s): To investigate OTP expression in 37 different tumor types, and the association between OTP expression and DNA methylation levels in lung neuroendocrine neoplasms.

Materials and methods: We analysed publicly available multi-omics data (whole-exome-, whole-genome-, RNA sequencing, Epic-850K-methylation array) of 58 typical-, 27 atypical carcinoids, 69 large cell neuroendocrine carcinomas and 51 small cell lung cancers and TCGA (The Cancer Genome Atlas) data of 33 tumor types. 850K-methylation analysis was cross-validated using targeted pyrosequencing on 35 carcinoids.

Conference:

Presenting Author: Moonen L

Authors: Moonen L, Mangiante L, Leunissen D, Gabriel A, Hillen L,

Keywords: Pulmonary, neuroendocrine, carcinoid, DNA methylation, Orthopedia Homeobox,