Comparative Expression Analysis of Chromosome 18 Related miRNAs in Ileal NET with and without Chr18 Loss

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Introduction: MicroRNAs (miRs) play important roles in many kinds of biological processes. Because the (partial) loss of one chromosome 18 (Chr18) is a frequent event in ileal NETs (iNETs), we assessed the potential deregulation of miR-expression in these patients.

Aim(s): We investigated the expression of 15 miRs localized on Chr18, in iNETs with and w/o Chr18 loss. For a robust quantification of miRs in NETs, we wanted to identify the best endogenous control for expression studies in iNETs and pancreatic NETs (pNETs).

Materials and methods: MiR-expression in FFPE iNETs (10 with, 10 w/o Chr18 loss) was analyzed by real-time PCR. To identify the best normalizer, nine small RNAs were tested in 10 ileal NETs. The stability of expression of the best three RNAs was further confirmed in 10 pNETs. Data analysis was obtained using the ΔΔCp comparative Cp method, significance values were determined by Mann-Whitney test.

Conference: 10th Annual ENETSConcerence (2013)

Presenting Author: Sperveslage J

Authors: Hoffmeister M, Sperveslage J, Henopp T, Anlauf M, Petersen K,

Keywords: NET, mir-expression,