Exploring decorin in plasma-derived small extracellular vesicles (sEVs) as a potential diagnostic biomarker for pancreatic neuroendocrine tumours

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Introduction: Pancreatic Neuroendocrine tumours (PanNETs), the most common site for GEP‑NENs in Asian countries, particularly India, have shown increasing incidence. Due to a lack of specific biomarkers, often detected at advanced stages. So, this study utilised PanNET tissue proteomics for cancer biomarker discovery and explored the impact of small extracellular cellular vesicles (sEVs) in PanNET growth and metastasis, as they reflect the molecular profile of their tissue of origin.

Aim(s): To perform a comprehensive proteomic analysis of tissue of PanNET and healthy controls (HCs) and to correlate these findings in plasma derived sEVs.

Materials and methods: Proteomic analysis of 6 PanNET and 3 HCs tissue samples was performed. Data analysis was performed using MetaboAnalyst 5.0, with validation through immunofluorescence. Isolation of sEV were done by precipitation method. Characterisation and validation of isolated sEVs (26 PanNETs and 25 HCs) was done by TEM, NTA and western blot. Dysregulated proteins were further validated in plasma and plasma derived sEVs.

Conference:

Presenting Author:

Authors: Gorai P, As S, Naik M, Kumar S, Pal S,

Keywords: Decorin, small extracellular vesicles, Pancreatic Neuroendocrine Tumour, Proteomics, circulating biomarker,