Exploring the interplay between RNA methylation and splicing dysregulation in neuroendocrine tumours

#4559

Introduction: Post-transcriptional modifications play crucial roles in regulating gene expression and cellular function. While recent findings indicate that alternative splicing dysregulation plays a key role in tumorigenic properties of neuroendocrine tumours (NETs), the role of other RNA processing mechanisms, such as N6-methyladenosine (m6A) — being the most common RNA methylation — remains largely unexplored in NETs.

Aim(s): We hypothesised that m6A processing machinery may be altered in NETs and linked to splicing dysregulation, thus RNA metabolism would be severely modified in these tumours. Our main aim was to identify alterations in the m6A process coupled to splicing alterations that may interfere with lung and pancreatic NETs development and aggressiveness.

Materials and methods: To this end, we have carried out a biocomputational analysis in two cohorts comprising 281 lung and 174 pancreatic NETs, exploring putative associations between m6A regulators with clinical features and splicing alterations.

Conference:

Presenting Author:

Authors: Ruiz-Palacios D, Gutiérrez Camacho L, García Vioque V, Moreno Montilla M, Blázquez-Encinas R,

Keywords: RNA methylation, splicing, neuroendocrine tumour, lung NET, pan NET,