Fluorescence labelled plasma derived small extracellular vesicle as biomarkers in metastatic pancreatic neuroendocrine tumour patients

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Introduction: Pancreatic neuroendocrine tumours (PanNET) are rare with increased annual incidence rate 1/lac. There are no specific biomarkers, diagnostic and prognostic tests available for PanNET. The small extracellular vesicles (sEV) play a key role in tumour growth and metastasis and can be a potential biomarker for metastasis of PanNET.

Aim(s): To evaluate the morphology and concentration of plasma derived sEV of metastatic, non-metastatic PanNET patients (PanNETs) and age matched Healthy Control subjects (HCs).

Materials and methods: The Plasma sEV were isolated by chemical precipitation combined with ultrafiltration from subjects (17 metastatic PanNETs, 6 non-metastatic PanNETs and 10 HCs) followed by antibody-based validation with Tumor Suppressor Gene TSG 101, Fotillin-1, Glutamic Acid Decarboxylase GAD65, β actin and calnexin by Western blot (WB). Morphological characterisation of the sEV was done by transmission electron microscopy (TEM). The quantification of sEV was achieved by Nanoparticle tracking analyser (NTA) using scatter and fluorescence (lipid-binding dye-labelled) modes of NTA and further validation by antibody-based NTA.

Conference:

Presenting Author:

Authors: As S, Gorai P, Naik M, Kumar R, Kumar S,

Keywords: Metastasis, Pancreatic neuroendocrine tumour, Small extracellular vesicles, Fluorescence, Nanoparticle tracking analysis,