HIF-1a Determines the Metastatic Potential of the GEP-NET Cell Line BON-1


Introduction: Intratumoral hypoxia is a hallmark of solid tumor formation and a negative predictor of patient survival. Adaptation to hypoxia is mainly achieved by the transcription factor HIF-1a, which is upregulated in a diverse range of human and experimental tumors and their metastases. HIF-1a target genes have been implicated in the induction of invasion and metastasis. However, HIF-1a’s tumor-supporting action depends on cell type and microenvironment and the precise role of HIF-1a for the pathogenesis of neuroendocrine tumors (NET) is largely unknown.

Aim(s): Molecular analysis of HIF-1a’s role in the pathobiology of gastroenteropancreatic neuroendocrine tumors (GEP-NET).

Materials and methods: Functional inactivation of HIF-1a was achieved by lentiviral-mediated siRNA transduction. Substrate-independent growth was analyzed by colony formation assay. Migration capacity was examined via wound healing assays.

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Freitag de Molina K, Rohwer N, Detjen K, Wiedenmann B, Cramer T,

Keywords: hypoxia, hif-1a, metastases,

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