The Neuroendocrine Phenotype, Genomic Profile, and Therapeutic Sensitivity of GEPNET Cell Lines

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Introduction: Patient tumour-derived cell lines have been widely used for studying the molecular mechanisms of tumours and their response to therapy. The establishment of cell lines from gastro-entero-pancreatic neuroendocrine tumours (GEPNETs) has proved difficult, but despite the challenges a limited amount of cell lines now exists.

Aim(s): In this study, we aimed to define the neuroendocrine phenotype, molecular characteristics, and the therapeutic sensitivity of frequently used GEPNET cell lines.

Materials and methods: Immunophenotyping, copy-number profiling, whole-exome sequencing, and an inhibitor screening with over 1,200 inhibitors was performed on seven GEP-NET cell lines. Furthermore, primary cells were established from patient GEPNETs collected at surgery to evaluate the effect of MEKi and HDACi.

Conference: 15th Annual ENETSConcerence (2018)

Presenting Author:

Authors: Hofving T, Arvidsson Y, Almobarak B, Inge L, Pfragner R,

Keywords: neuroendocrine tumor, GEP-NET, immunophenotyping, copy-number alterations, exome sequencing, inhibitor screening, vorinostat, trametinib,

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