Distribution of T-Cell Infiltrate in G1, G2 and G3 NENs

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Introduction: The identification of T-cell infiltrate and the expression of inhibitory molecules in the tumor microenvironment could lend support to the use of immunotherapy in the treatment of gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs).

Aim(s): Study of T-Cell infiltrate in GEP NENs.

Materials and methods: Immunohistochemistry for CD3, CD4,CD8, HLA1, HLA DR and PDL1 was performed in primary and metastatic lesions from 52 patients with GEP-NENs classified according to ENETS/WHO 2010 as follow: 20 NET G1, 20 NET G2 and 12 NEC G3 and 39 Stage IV and 13 Stage IIIb). The expression (E) of each antibody was defined as follows: 1+, immunoreactivity in <25% of neoplastic cells; 2+, 26–50%; 3+, 51–75%; 4+, 76–100%. Intensity (I) was ranked as low (1+), normal (2+), or strong (3+), as compared with internal controls. E and I were combined into a single score (E×I). IHC was positive if score >1.

Conference: 13th Annual ENETSConcerence (2016)

Presenting Author: Milione M

Authors: Milione M, Pellegrinelli A, Centonze G, Dominoni F, Pusceddu S,

Keywords: Ki-67, T-cells, NEN,