Cancer-Associated-Fibroblasts in Neuroendocrine Neoplasms: A Role in Cancer Progression

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Introduction: Cancer-associated-fibroblasts (CAFs) secrete soluble factors that enhance tumor growth and invasion. Neuroendocrine tumor (NET) cells recruit fibroblasts within the lesional sites.

Aim(s): CAFs affect NET cells transcriptional profile thus postulating their hyperactive cross-talk with stroma.

Materials and methods: We established cultures of CAFs from enterochromaffin cell (ECL) hyperplasia, G1, G2, G3 NETs. Fibroblasts from perilesional tissue of NETs and WI-38 were used for control. Adherent cells were immunostained for CD34, CD45, CD56, CD73, CD90 and CD105. H720, H727, H835, BON1, CM and QGP1 NET cells were cultured with CAF conditioned medium (CM); their viability was assessed by MTS test while their proliferation by CFSE in the presence or not of inserts and by flow-cytometry.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author: Cives M

Authors: Cives M, Simone V, Rizzo F, Bisceglia F, Vecchio M,

Keywords: CAF, microenvironment, neuroendocrine tumors ,