Integrating Saffron’s Metabolomics on Neuroblastoma Treatment

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Introduction: Crocus sativus (saffron) is endowed with anticancer, antioxidant and genoprotective properties. Its pharmacological properties are attributed to the hydrophilic carotenoids crocins (CRCs), which are glycosy-esters of crocetin (CRT). The hemisynthetic derivate dimethylcrocetin (DMCRT) is similar to CRT.

Aim(s): The current research studies the in vitro effect of CRCs and DMCRT on neuroblastoma cell lines.

Materials and methods: CRCs are diluted to water and DMCRT to 10% DMSO. SH-SY5Y and BE(2)-M17 neuroblastoma cells are incubated at a range of concentrations of CRCs and DMCRT for 24, 48 and 72 hours. Analysis of cell viability is performed via Alamar Blue assay. Real-Time PCR assesses alterations in the expression of genes, implicated in neuroblastoma initiation and cell apoptosis.

Conference:

Presenting Author:

Authors: Hatziagapiou K, Nikola O, Koniari E, Chrousos G, Kanaka-Gantenbein C,

Keywords: saffron, crocins, dimethylocrocetin, neuroblastoma, cytotoxicity, apoptosis,