Reduced MEN1 Gene Expression in Pulmonary Carcinoids Is Associated With Metastatic Disease

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Introduction: Lung carcinoids are neuroendocrine tumors that can be classified as typical (TC) or atypical (AC) carcinoids. Previously, MEN-1 mutations and associated LOH of 11q13 have been implied in tumorigenesis.

Aim(s): To examine MEN-1 promotor hypermethylation and gene expression and their correlation with clinical outcome.

Materials and methods: We determined MEN-1 mRNA expression in 20 ACs and 31 TCs by quantitative RT-PCR (exon 2/3 boundaries). Furthermore, we analyzed genomic DNA from 25 ACs and 22 TCs for MEN-1 promotor hypermethylation by Methylation Specific PCR. Results were compared to controls (normal tissues and neuroendocrine cell lines) and correlated with clinical outcome.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Swarts D, Henfling M, Ramaekers F, Van Suylen R, Dingemans A,

Keywords: bronchial carcinoids, methylation specific PCR, multiple endocrine neoplasia type 1, hypermethylation,