TIM16 Inhibition Decreases Calcitonin Secretion and Enhances Sensitivity to Paclitaxel in a Human Medullary Thyroid Carcinoma Cells

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Introduction: TIM 16 protein is part of the traslocase complex TIM 23 situated in the mitochondrial inner membrane, encoded by the Magmas gene, that is overexpressed in several tumors. Magmas silencing causes a greater sensitivity to apoptotic stimuli. We recently demonstrated that, in a human medullary thyroid carcinoma cell line (TT), compound 5, a TIM 16 inhibitor, was not cytotoxic but enhanced the proapoptotic effects of staurosporine

Aim(s): To verify whether compound 5 influences calcitonin secretion and cytotoxic effect of paclitaxel

Materials and methods: Cell viability and apoptotic activation were evaluated by ATPlite assay and Caspase 3/7 assay, respectively. ELISA test was used for calcitonin detection in cell culture medium

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author:

Authors: Gagliano T, Riva E, Tagliati F, Matteotti D, Brugnoli V,

Keywords: TIM16, Magmas, Paclitaxel, Calcitonin, Medullary Thyroid Carcinoma,