Integrative molecular analysis of lung neuroendocrine neoplasms with different Ki-67 indices identifies a molecular transition group between low- and high-grade neoplasms

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Introduction: In the 2021 World Health Organization (WHO) classification of lung cancers, lung neuroendocrine neoplasms (LNENs) are classified as low-grade, intermediate-grade and high-grade based on mitotic count but not Ki-67 index, as for GEP-NENs.

Aim(s): In order to test a hypothetical Ki-67-based classification supported by genomic and transcriptomic data, we performed the molecular characterization of a well-known series of LNETs with a very broad range of Ki67 indices.

Materials and methods: For this purpose, 126 LNETs were assessed for transcriptomic profiling of 20,815 genes and genomic alterations in 409 genes. Two approaches were used: 1) supervised, where samples were grouped in 4 categories (G1, G2, G3 and NEC) according to Ki-67 index, and 2) unsupervised.

Conference:

Presenting Author:

Authors: Simbolo M, Centonze G, Ali G, Grillo F, Sabella G,

Keywords: lung neuroendocrine tumor, NET grade 3, next generation sequencing, transcriptomics,