Abstract Library

#3796 The effect of pseudohypoxia on the immune microenvironment of pancreatic neuroendocrine neoplasms (PanNEN)

Introduction: Pancreatic neuroendocrine tumors may develop sporadically or as part of an inherited disease, such as von Hippel-Lindau (VHL). VHL disease is caused by a germline pathogenic variant in the VHL gene encoding VHL protein (pVHL). Hypoxia inducible factor (HIF) is responsible for cellular oxygen supply. Its degradation is mediated in normoxic states via ubiquitination and inhibited by pVHL. Hence, pVHL deficiency leads to pseudohypoxia due to HIF overexpression. Several studies suggested immunomodulatory role for HIF in kidney cancer.

Conference:

Presenting Author:

Authors: Solomon Chmelnik A, Telerman A, Tirosh A,

Keywords: Pancreatic neuroendocrine tumor, VHL, DNA methylation, Immune cells, PD-L1,

#3387 High tissue expression of Flt3L is linked to higher infiltration of immune cells in patients with high-grade GEP-NEN

Introduction: The clinical management of gastroenteropancreatic neuroendocrine neoplasms (GEP-NEN) remains challenging due to disease heterogeneity. We have recently shown that mRNA expression of the immunostimulatory cytokine FMS-like tyrosine kinase 3 ligand (Flt3L) in tumor tissue is a prognostic biomarker in high-grade GEP-NEN patients. However, a direct link between Flt3L expression and the presence of proinflammatory immune cells in the tumor microenvironment (TME) of GEP-NEN is still missing.

Conference:

Presenting Author: Werner W

Authors: Werner W, Detjen K, Bruneau A, Wiedenmann B, Tacke F,

Keywords: NET, Flt3L, DC, dendritic cell, biomarker, immune response, immune microenvironment,

#3097 Infiltrating pattern and prognostic value of tertiary lymphoid structures in pancreatic neuroendocrine tumors

Introduction: Tertiary lymphoid structures (TLS) are associated with favourable survival and play a critical role in most solid tumors. However, investigations of TLS are lacking in grade 1 or grade 2 (G1/G2) non-functional pancreatic neuroendocrine tumor (NF-PanNET) patients.

Conference: 18th Annual ENETS Concerence (2021)

Presenting Author: Zhang W

Authors: Zhang W, Wang W, Han X, Gao H, Yu X,

Keywords: non-functional pancreatic neuroendocrine tumors, tertiary lymphoid structures, tumor immune microenvironment, prognosis, nomogram,

#2827 Characterization of Epigenetic Modulation in Pancreatic Neuroendocrine Neoplasms

Introduction: Pancreatic neuroendocrine tumors (PanNETs) represent a rare and heterogeneous tumor entity. Despite advances in therapy cancer progression and resistance mechanisms are still challenging. Recent genome wide sequencing analyses in PanNETs identified a large number of mutated genes involved in epigenetic modulation.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Schmitz R

Authors: Schmitz R, Weißbach J, Kleinlein J, Schrader J, Grabowski P,

Keywords: PanNET, HDAC-Inhibition, SOCS3, lncRNA, Macrophages,

#2810 Development of a Personalized Human Immunocompetent Ex-Vivo Model of Neuroendocrine Liver Metastasis Using Precision Cut Tissue Slice Technology

Introduction: Liver metastasis (LM) are very frequent (65-95%) and represent a crucial prognostic factor in patients with gastroenteropancreatic neuroendocrine neoplasms (GEP NENs). The paucity of relevant experimental models has limited the understanding of the pathophysiology of NENs and the development of efficient therapeutic strategies

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Doornebal E

Authors: Doornebal E, Harris N, Miquel R, Zen Y, Pizanias M,

Keywords: Liver, neuroendocrine liver metastasis, ex-vivo model, personalized medicine, immunocompetent, organotypic tissue slices,