Abstract Library

#1850 Influence of VEGF Splicing on Microvessel Density and Architecture in Pancreatic Neuroendocrine Tumours

Introduction: Hypoxia in human tumours is associated with a poor prognosis and is characterized by the stabilization of HIF1α, upregulation of VEGF, and a high microvessel density (MVD). Interestingly, although pancreatic neuroendocrine tumours (pNETS) showing hypoxia signalling also have a poor prognosis, they paradoxically display a low MVD. We hypothesized that this paradoxon might be explained by alternative splicing of VEGF.

Conference: 14th Annual ENETSConcerence (2017)

Presenting Author: Schmitt A

Authors: Marinoni I, Naim S, Centeno I, Perren A, Schmitt A,

Keywords: pNET, VEGF,

#1104 Interlaboratory Variability of MIB1 Staining in Well Differentiated Pancreatic Neuroendocrine Tumors

Introduction: In neuroendocrine tumors (NET) grading and staging are the only routinely performed procedures to judge prognosis. Proliferation index using MIB1 staining has been introduced to assess grading. There are discussions on cut-off definitions, automated counting and inter-observer variability.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author: Perren A

Authors: Blank A, Wehweck L, Marinoni I, Boos L, Schmitt A,

Keywords: MIB1, Ki67, grading, NET,

#856 DAXX and ATRX Loss Defines Chromosomal Instability and Poor Outcome in Pancreatic NET

Introduction: Chromosomal Instability (CIN) has been reported in pancreatic neuroendocrine tumors (pNETs) with poor outcome. However, no specific genetic background has been associated with CIN. Whole exome sequencing revealed mutations in DAXX (Death domain associated protein gene) and ATRX (ATR-X gene) in 40% of pNETs. DAXX and ATRX mutations in pNETs are associated with Alternative Lengthening of Telomeres (ALT) activation.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author:

Authors: Marinoni I, Schmitt A, Vassella E, Dettmer M, Rudolph T,

Keywords: pNET, Daxx, Atrx, ALT, CIN,

#246 SDHB Loss Predicts Malignancy in Pheochromocytomas/Sympathethic Paragangliomas, but Not Through Hypoxia Signalling

Introduction: To date there is no reliable histopathological marker of malignancy for pheochromocytomas/sympathetic paragangliomas (PCC/PGL). It is well-known that PCC/PGL in the hereditary context of an SDHB germline mutation very often metastasize. The immunohistochemical loss of SDHB expression was recently shown to be a surrogate marker for the presence of an SDH germline mutation in PCC/PGL. SDHB loss is supposed to be tumorigenic via activation of hypoxia signals.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Schmitt A, Blank A, Korpershoek E, van Nederveen F, Rudolph T,

Keywords: SDHB loss, immunohistochemistry, pheochromocytoma, paraganglioma, malignancy, hypoxia,

#171 Transcription Factor Signature in Pancreatic and Duodenal Neuroendocrine Neoplasms

Introduction: We recently identified ISL1 as a marker for pancreatic neuroendocrine neoplasms (P-NENs). ISL1 as well as PDX1, CDX2 and NGN3 are transcription factors (TF) involved in pancreato-duodenal development.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Hermann G, Klöppel G, Konukiewitz B, Schmitt A, Perren A,

Keywords: neuroendocrine, neoplasm, pancreas, duodenum, transcription factors, isl1, pdx1, ngn3, cdx2, ihc,